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GenScript corporation online pcr primers designs tool
Online Pcr Primers Designs Tool, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/online+pcr+primers+designs+tool/online+pcr+primers+designs+tool/10__3390_slash_ijms26073429-424-7-12
Average 90 stars, based on 1 article reviews
online pcr primers designs tool - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Growth, health, and immune status of Nile tilapia Oreochromis niloticus cultured at different stocking rates and fed algal β-carotene
Article Snippet: .. The primers were designed using the software GenScript Online PCR Primers Designs Tool. β-actin and 18 s rRNA were used as a housekeeping gene. .. Quantitative PCR reaction contained 2.5 μL of 1 μg/μL cDNA, 12.5 μL SimplyGreen SYBR Green qPCR Master Mix, Low Rox (Cat SQ102–0100, GeneDireX, Inc), 0.3 μM of each of forward and reverse primers, 1 μL RNase inhibitor and RNase-Free water to a final volume of 25 μL.

Article Title: Schistosoma mansoni FES Tyrosine Kinase Involvement in the Mammalian Schistosomiasis Outcome and Miracidia Infection Capability in Biomphalaria glabrata
Article Snippet: .. The software Primer 3 and GenScript Online PCR Primers Designs Tool were used for primer design. ..

Polymerase Chain Reaction:

Article Title: Growth, health, and immune status of Nile tilapia Oreochromis niloticus cultured at different stocking rates and fed algal β-carotene
Article Snippet: .. The primers were designed using the software GenScript Online PCR Primers Designs Tool. β-actin and 18 s rRNA were used as a housekeeping gene. .. Quantitative PCR reaction contained 2.5 μL of 1 μg/μL cDNA, 12.5 μL SimplyGreen SYBR Green qPCR Master Mix, Low Rox (Cat SQ102–0100, GeneDireX, Inc), 0.3 μM of each of forward and reverse primers, 1 μL RNase inhibitor and RNase-Free water to a final volume of 25 μL.

Article Title: A metagenomics approach to identification of eukaryotes in metazoan-associated microbiomes
Article Snippet: .. Primers were constructed using GenScript Online PCR Primers Designs Tool ( https://www.genscript.com/tools/pcr-primers-designer ). .. Primers produced a 200 base pair (bp) amplicon from these diagnostic loci. qPCR was performed from extracted DNA using an Applied Biosystems QuantStudio 5 Real-Time PCR System with PowerUp SYBR Green Master Mix (Applied Biosystems, Foster City, CA).

Article Title: Magnetic Nanoparticle‐Assisted Non‐Viral CRISPR‐Cas9 for Enhanced Genome Editing to Treat Rett Syndrome
Article Snippet: .. All primer sequences were designed using GenScript Online PCR Primers Designs Tool. ..

Article Title: Genome-Wide Identification of the BTB Domain-Containing Protein Gene Family in Pepper ( Capsicum annuum L.)
Article Snippet: .. These primers were designed using the GenScript Online PCR Primers Designs Tool ( https://www.genscript.com/tools/pcr-primers-designer , accessed on 15 January 2025). ..

Article Title: Improvement of crAssphage detection/quantification method and its extensive application for food safety
Article Snippet: .. For conventional PCR, the DNA sequences of a For/Rev18-F primer set were obtained from the previous study , and CrAssORF24 and CrAssORF44 primer sets were designed using Online PCR Primers Designs Tool (GenScript, United States). ..

Article Title: Genome-Wide Identification of the BTB Domain-Containing Protein Gene Family in Pepper (Capsicum annuum L.)
Article Snippet: .. These primers were designed using the GenScript Online PCR Primers Designs Tool (https://www.genscript.com/tools/pcr-primers-designer, accessed on 15 January 2025). ..

Article Title: Upregulation of TRESK Channels Contributes to Motor and Sensory Recovery after Spinal Cord Injury
Article Snippet: Changes in TRESK mRNA expression in the brain, spinal cord, and DRG following the SCI were quantified using real-time PCR with FastStart DNA Master SYBR Green I (Roche Applied Science, Mannheim, Germany) and the LightCycler System (LightCycler 2.0 instrument, Roche). .. Real-time PCR primers were designed using GenScript Online PCR Primers Designs Tool (GenScript, Piscataway, NJ, USA). ..

Article Title: Schistosoma mansoni FES Tyrosine Kinase Involvement in the Mammalian Schistosomiasis Outcome and Miracidia Infection Capability in Biomphalaria glabrata
Article Snippet: .. The software Primer 3 and GenScript Online PCR Primers Designs Tool were used for primer design. ..

Construct:

Article Title: A metagenomics approach to identification of eukaryotes in metazoan-associated microbiomes
Article Snippet: .. Primers were constructed using GenScript Online PCR Primers Designs Tool ( https://www.genscript.com/tools/pcr-primers-designer ). .. Primers produced a 200 base pair (bp) amplicon from these diagnostic loci. qPCR was performed from extracted DNA using an Applied Biosystems QuantStudio 5 Real-Time PCR System with PowerUp SYBR Green Master Mix (Applied Biosystems, Foster City, CA).

Real-time Polymerase Chain Reaction:

Article Title: Upregulation of TRESK Channels Contributes to Motor and Sensory Recovery after Spinal Cord Injury
Article Snippet: Changes in TRESK mRNA expression in the brain, spinal cord, and DRG following the SCI were quantified using real-time PCR with FastStart DNA Master SYBR Green I (Roche Applied Science, Mannheim, Germany) and the LightCycler System (LightCycler 2.0 instrument, Roche). .. Real-time PCR primers were designed using GenScript Online PCR Primers Designs Tool (GenScript, Piscataway, NJ, USA). ..



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